Composite

Part:BBa_K1615077:Design

Designed by: Brooke Rothschild-Mancinelli   Group: iGEM15_Edinburgh   (2015-09-02)


Linker-CBDcipA-linker fused to TVEL5 in RFC25


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1329
    Illegal BglII site found at 1490
    Illegal BamHI site found at 1553
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1135


Design Notes

Fusion done using RFC25.


Source

TVEL5 Laccase from Tramates versicolor. CBDcipA from Clostridium thermocellum.

References